The Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late Blight.
Identifieur interne : 000912 ( Main/Exploration ); précédent : 000911; suivant : 000913The Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late Blight.
Auteurs : Paul Dahlin [Suède] ; Marion C. Müller [Suède] ; Sophia Ekengren [Suède] ; Lauren S. Mckee [Suède] ; Vincent Bulone [Suède]Source :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2017.
Descripteurs français
- KwdFr :
- Alcaloïdes des Solanaceae (composition chimique), Alcaloïdes des Solanaceae (pharmacologie), Diosgénine (composition chimique), Diosgénine (pharmacologie), Glycosidases (génétique), Glycosidases (métabolisme), Glycosylation (MeSH), Interactions hôte-pathogène (effets des médicaments et des substances chimiques), Lycopersicon esculentum (microbiologie), Maladies des plantes (microbiologie), Mycelium (effets des médicaments et des substances chimiques), Mycelium (génétique), Mycelium (physiologie), Phytophthora infestans (effets des médicaments et des substances chimiques), Phytophthora infestans (génétique), Phytophthora infestans (physiologie), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Solanine (analogues et dérivés), Solanine (composition chimique), Solanine (pharmacologie), Solanum tuberosum (microbiologie), Structure moléculaire (MeSH), Stéroïdes (composition chimique), Stéroïdes (pharmacologie), Séquence glucidique (MeSH).
- MESH :
- analogues et dérivés : Solanine.
- composition chimique : Alcaloïdes des Solanaceae, Diosgénine, Solanine, Stéroïdes.
- effets des médicaments et des substances chimiques : Interactions hôte-pathogène, Mycelium, Phytophthora infestans.
- génétique : Glycosidases, Mycelium, Phytophthora infestans, Protéines fongiques.
- microbiologie : Lycopersicon esculentum, Maladies des plantes, Solanum tuberosum.
- métabolisme : Glycosidases, Protéines fongiques.
- pharmacologie : Alcaloïdes des Solanaceae, Diosgénine, Solanine, Stéroïdes.
- physiologie : Mycelium, Phytophthora infestans.
- Glycosylation, Structure moléculaire, Séquence glucidique.
English descriptors
- KwdEn :
- Carbohydrate Sequence (MeSH), Diosgenin (chemistry), Diosgenin (pharmacology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Glycoside Hydrolases (genetics), Glycoside Hydrolases (metabolism), Glycosylation (MeSH), Host-Pathogen Interactions (drug effects), Lycopersicon esculentum (microbiology), Molecular Structure (MeSH), Mycelium (drug effects), Mycelium (genetics), Mycelium (physiology), Phytophthora infestans (drug effects), Phytophthora infestans (genetics), Phytophthora infestans (physiology), Plant Diseases (microbiology), Solanaceous Alkaloids (chemistry), Solanaceous Alkaloids (pharmacology), Solanine (analogs & derivatives), Solanine (chemistry), Solanine (pharmacology), Solanum tuberosum (microbiology), Steroids (chemistry), Steroids (pharmacology).
- MESH :
- chemical , analogs & derivatives : Solanine.
- chemical , chemistry : Diosgenin, Solanaceous Alkaloids, Solanine, Steroids.
- chemical , genetics : Fungal Proteins, Glycoside Hydrolases.
- chemical , metabolism : Fungal Proteins, Glycoside Hydrolases.
- chemical , pharmacology : Diosgenin, Solanaceous Alkaloids, Solanine, Steroids.
- drug effects : Host-Pathogen Interactions, Mycelium, Phytophthora infestans.
- genetics : Mycelium, Phytophthora infestans.
- microbiology : Lycopersicon esculentum, Plant Diseases, Solanum tuberosum.
- physiology : Mycelium, Phytophthora infestans.
- Carbohydrate Sequence, Glycosylation, Molecular Structure.
Abstract
Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity are sterol-mediated disruption of membranes and inhibition of cholinesterase activity in neurons. It has been suggested that phytopathogenic microorganisms can overcome SGA toxicity by enzymatic deglycosylation of SGAs. Here, we have explored SGA-mediated toxicity toward the invasive oomycete Phytophthora infestans, the causative agent of the late blight disease in potato and tomato, as well as the potential for SGA deglycosylation by this species. Our growth studies indicate that solanidine, the nonglycosylated precursor of the potato SGAs α-chaconine and α-solanine, has a greater physiological impact than its glycosylated forms. All of these compounds were incorporated into the mycelium, but only solanidine could strongly inhibit the growth of P. infestans in liquid culture. Genes encoding several glycoside hydrolases with potential activity on SGAs were identified in the genome of P. infestans and were shown to be expressed. However, we found no indication that deglycosylation of SGAs takes place. We present additional evidence for apparent host-specific adaptation to potato SGAs and assess all results in terms of future pathogen management strategies.
DOI: 10.1094/MPMI-09-16-0186-R
PubMed: 28510502
Affiliations:
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Müller</name><affiliation wicri:level="1"><nlm:affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName><orgName type="university">Université de Stockholm</orgName><placeName><settlement type="city">Stockholm</settlement><region nuts="1">Svealand</region><region nuts="1">Comté de Stockholm</region></placeName></affiliation></author><author><name sortKey="Ekengren, Sophia" sort="Ekengren, Sophia" uniqKey="Ekengren S" first="Sophia" last="Ekengren">Sophia Ekengren</name><affiliation wicri:level="1"><nlm:affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName><orgName type="university">Université de Stockholm</orgName><placeName><settlement type="city">Stockholm</settlement><region nuts="1">Svealand</region><region nuts="1">Comté de Stockholm</region></placeName></affiliation></author><author><name sortKey="Mckee, Lauren S" sort="Mckee, Lauren S" uniqKey="Mckee L" first="Lauren S" last="Mckee">Lauren S. Mckee</name><affiliation wicri:level="1"><nlm:affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation><nlm:affiliation>3 Wallenberg Wood Science Centre, Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden; and.</nlm:affiliation><wicri:noCountry code="subField">Sweden; and</wicri:noCountry></affiliation></author><author><name sortKey="Bulone, Vincent" sort="Bulone, Vincent" uniqKey="Bulone V" first="Vincent" last="Bulone">Vincent Bulone</name><affiliation wicri:level="1"><nlm:affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation><nlm:affiliation>4 ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064 Australia.</nlm:affiliation><wicri:noCountry code="subField">SA 5064 Australia</wicri:noCountry></affiliation></author></analytic><series><title level="j">Molecular plant-microbe interactions : MPMI</title><idno type="ISSN">0894-0282</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbohydrate Sequence (MeSH)</term><term>Diosgenin (chemistry)</term><term>Diosgenin (pharmacology)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Glycoside Hydrolases (genetics)</term><term>Glycoside Hydrolases (metabolism)</term><term>Glycosylation (MeSH)</term><term>Host-Pathogen Interactions (drug effects)</term><term>Lycopersicon esculentum (microbiology)</term><term>Molecular Structure (MeSH)</term><term>Mycelium (drug effects)</term><term>Mycelium (genetics)</term><term>Mycelium (physiology)</term><term>Phytophthora infestans (drug effects)</term><term>Phytophthora infestans (genetics)</term><term>Phytophthora infestans (physiology)</term><term>Plant Diseases (microbiology)</term><term>Solanaceous Alkaloids (chemistry)</term><term>Solanaceous Alkaloids (pharmacology)</term><term>Solanine (analogs & derivatives)</term><term>Solanine (chemistry)</term><term>Solanine (pharmacology)</term><term>Solanum tuberosum (microbiology)</term><term>Steroids (chemistry)</term><term>Steroids (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alcaloïdes des Solanaceae (composition chimique)</term><term>Alcaloïdes des Solanaceae (pharmacologie)</term><term>Diosgénine (composition chimique)</term><term>Diosgénine (pharmacologie)</term><term>Glycosidases (génétique)</term><term>Glycosidases (métabolisme)</term><term>Glycosylation (MeSH)</term><term>Interactions hôte-pathogène (effets des médicaments et des substances chimiques)</term><term>Lycopersicon esculentum (microbiologie)</term><term>Maladies des plantes (microbiologie)</term><term>Mycelium (effets des médicaments et des substances chimiques)</term><term>Mycelium (génétique)</term><term>Mycelium (physiologie)</term><term>Phytophthora infestans (effets des médicaments et des substances chimiques)</term><term>Phytophthora infestans (génétique)</term><term>Phytophthora infestans (physiologie)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Solanine (analogues et dérivés)</term><term>Solanine (composition chimique)</term><term>Solanine (pharmacologie)</term><term>Solanum tuberosum (microbiologie)</term><term>Structure moléculaire (MeSH)</term><term>Stéroïdes (composition chimique)</term><term>Stéroïdes (pharmacologie)</term><term>Séquence glucidique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Solanine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Diosgenin</term><term>Solanaceous Alkaloids</term><term>Solanine</term><term>Steroids</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Glycoside Hydrolases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Diosgenin</term><term>Solanaceous Alkaloids</term><term>Solanine</term><term>Steroids</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Solanine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alcaloïdes des Solanaceae</term><term>Diosgénine</term><term>Solanine</term><term>Stéroïdes</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Host-Pathogen Interactions</term><term>Mycelium</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Interactions hôte-pathogène</term><term>Mycelium</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mycelium</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycosidases</term><term>Mycelium</term><term>Phytophthora infestans</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Lycopersicon esculentum</term><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Lycopersicon esculentum</term><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycosidases</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alcaloïdes des Solanaceae</term><term>Diosgénine</term><term>Solanine</term><term>Stéroïdes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Mycelium</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycelium</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Sequence</term><term>Glycosylation</term><term>Molecular Structure</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Glycosylation</term><term>Structure moléculaire</term><term>Séquence glucidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity are sterol-mediated disruption of membranes and inhibition of cholinesterase activity in neurons. It has been suggested that phytopathogenic microorganisms can overcome SGA toxicity by enzymatic deglycosylation of SGAs. Here, we have explored SGA-mediated toxicity toward the invasive oomycete Phytophthora infestans, the causative agent of the late blight disease in potato and tomato, as well as the potential for SGA deglycosylation by this species. Our growth studies indicate that solanidine, the nonglycosylated precursor of the potato SGAs α-chaconine and α-solanine, has a greater physiological impact than its glycosylated forms. All of these compounds were incorporated into the mycelium, but only solanidine could strongly inhibit the growth of P. infestans in liquid culture. Genes encoding several glycoside hydrolases with potential activity on SGAs were identified in the genome of P. infestans and were shown to be expressed. However, we found no indication that deglycosylation of SGAs takes place. We present additional evidence for apparent host-specific adaptation to potato SGAs and assess all results in terms of future pathogen management strategies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28510502</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>06</Month><Day>05</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>7</Issue><PubDate><Year>2017</Year><Month>07</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>The Impact of Steroidal Glycoalkaloids on the Physiology of Phytophthora infestans, the Causative Agent of Potato Late Blight.</ArticleTitle><Pagination><MedlinePgn>531-542</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-09-16-0186-R</ELocationID><Abstract><AbstractText>Steroidal glycoalkaloids (SGAs) are plant secondary metabolites known to be toxic to animals and humans and that have putative roles in defense against pests. The proposed mechanisms of SGA toxicity are sterol-mediated disruption of membranes and inhibition of cholinesterase activity in neurons. It has been suggested that phytopathogenic microorganisms can overcome SGA toxicity by enzymatic deglycosylation of SGAs. Here, we have explored SGA-mediated toxicity toward the invasive oomycete Phytophthora infestans, the causative agent of the late blight disease in potato and tomato, as well as the potential for SGA deglycosylation by this species. Our growth studies indicate that solanidine, the nonglycosylated precursor of the potato SGAs α-chaconine and α-solanine, has a greater physiological impact than its glycosylated forms. All of these compounds were incorporated into the mycelium, but only solanidine could strongly inhibit the growth of P. infestans in liquid culture. Genes encoding several glycoside hydrolases with potential activity on SGAs were identified in the genome of P. infestans and were shown to be expressed. However, we found no indication that deglycosylation of SGAs takes place. We present additional evidence for apparent host-specific adaptation to potato SGAs and assess all results in terms of future pathogen management strategies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dahlin</LastName><ForeName>Paul</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Marion C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ekengren</LastName><ForeName>Sophia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>2 Department of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McKee</LastName><ForeName>Lauren S</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>3 Wallenberg Wood Science Centre, Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 106 91 Stockholm, Sweden; and.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bulone</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>1 Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, 10691 Stockholm, Sweden.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>4 ARC Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, The University of Adelaide, Waite Campus, Urrbrae, SA 5064 Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>05</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Mol Plant Microbe Interact</MedlineTA><NlmUniqueID>9107902</NlmUniqueID><ISSNLinking>0894-0282</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012991">Solanaceous Alkaloids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013256">Steroids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C514919">alpha-solanine</NameOfSubstance></Chemical><Chemical><RegistryNumber>20562-02-1</RegistryNumber><NameOfSubstance UI="D012992">Solanine</NameOfSubstance></Chemical><Chemical><RegistryNumber>20562-03-2</RegistryNumber><NameOfSubstance UI="C011860">alpha-chaconine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D006026">Glycoside Hydrolases</NameOfSubstance></Chemical><Chemical><RegistryNumber>K49P2K8WLX</RegistryNumber><NameOfSubstance UI="D004144">Diosgenin</NameOfSubstance></Chemical><Chemical><RegistryNumber>W7801OHM8B</RegistryNumber><NameOfSubstance UI="C013255">solanidine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002240" MajorTopicYN="N">Carbohydrate Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004144" MajorTopicYN="N">Diosgenin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006026" MajorTopicYN="N">Glycoside Hydrolases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018551" MajorTopicYN="N">Lycopersicon esculentum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025282" MajorTopicYN="N">Mycelium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012991" MajorTopicYN="N">Solanaceous Alkaloids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012992" MajorTopicYN="N">Solanine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013256" MajorTopicYN="N">Steroids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28510502</ArticleId><ArticleId IdType="doi">10.1094/MPMI-09-16-0186-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country><region><li>Comté de Stockholm</li><li>Svealand</li></region><settlement><li>Stockholm</li></settlement><orgName><li>Université de Stockholm</li></orgName></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Dahlin, Paul" sort="Dahlin, Paul" uniqKey="Dahlin P" first="Paul" last="Dahlin">Paul Dahlin</name></region><name sortKey="Bulone, Vincent" sort="Bulone, Vincent" uniqKey="Bulone V" first="Vincent" last="Bulone">Vincent Bulone</name><name sortKey="Dahlin, Paul" sort="Dahlin, Paul" uniqKey="Dahlin P" first="Paul" last="Dahlin">Paul Dahlin</name><name sortKey="Ekengren, Sophia" sort="Ekengren, Sophia" uniqKey="Ekengren S" first="Sophia" last="Ekengren">Sophia Ekengren</name><name sortKey="Ekengren, Sophia" sort="Ekengren, Sophia" uniqKey="Ekengren S" first="Sophia" last="Ekengren">Sophia Ekengren</name><name sortKey="Mckee, Lauren S" sort="Mckee, Lauren S" uniqKey="Mckee L" first="Lauren S" last="Mckee">Lauren S. Mckee</name><name sortKey="Muller, Marion C" sort="Muller, Marion C" uniqKey="Muller M" first="Marion C" last="Müller">Marion C. Müller</name><name sortKey="Muller, Marion C" sort="Muller, Marion C" uniqKey="Muller M" first="Marion C" last="Müller">Marion C. Müller</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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